I am trying to find the optimal registration process for these two images. They are of the same tissue across different stains, which means that they have different RGB qualities but are of the same basic size. There is some tissue damage in the second image which is causing issues for me when I try ITK translation registration. My initial goal was to do a composite registration consisting of a translation or affine shift, followed by a Bspline transform to account for the micro-tissue movement. Any idea on if this is the best approach? I haven’t had much success.