Hi Lee,
In the meantime, I used a Python version of the original Macenko MATLAB code to see how it worked. It hard-codes the reference stain parameters (but I couldn’t find where they came from) and so is less appealing and less flexible than your package. But it allows me to see what is used in the normalized output for each input stain, hematoxylin and eosin, as attached.
Is there a way to get at these separated images in your package?
Cheers,
Jon
Jon R. Sauer
jon.sauer@gmail.com
Acton, MA, USA 01720
+1 303.579.3009
