Our lab is using the Demons algorithm through SimpleITK to register some microscopy images to a template. Right now the alignment take up up to 130 gigs of ram for 2 gig images and is rather slow. Also alignment results do not seem to improve beyond registering at the lowest resolution down sampling by a factor of four.
I am wondering if you have any intuition on how to adjust the parameter of the algorithm to achieve the results below: 1. reduce RAM usage. 2. decrease computation time. 3. increase alignment accuracy.
Thanks a lot!