how to preserve transform of full resolution image

Beginner Questions
1

I have a group of rgb tiff images that have been registered using low resolution tiles, I want to apply the transform to each tiff image save the image back to a new tiff file but with the full resolution and tiles etc, so that when I go to build an image volume I can decide then what resolution I want.

right I am using tifffile python to read the tiled image which lets me choose which tile/resolution I want for the registration piece, but maybe there is a better way to do this for the application of the transforms?

I can resample the rgb with the transform but am limited to the single resolution that I choose at load time

2

If your lower resolution tiles have appropriately larger pixel spacing, the transforms can remain the same. This is because ITK applies transforms (and does registration) in physical space, not index space.

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3

I believe that’s what I am trying to do but am not sure I can wrap my head around it.

Can I apply resampling in a way that converts every resolution the same or do I simply resample the highest transformed resolution image and saved it in tiled format. is anything lost?

-Kurt

4

This sounds like it would lead to least loss of information.

But you should be able to resample different resolutions with little change. Pseudocode:

set up transforms, resample filter etc.

for r in resolutions
  power2=2**r
  resampleFilter.SetInput("path"+r+".png")
  resampleFilter.SetTargetSpacing(originalSpacing*power2)
  resampleFilter.SetTargetSize(originalSpacing/power2)
  resampleFilter.execute
  writeImage(resampleFilter.output)
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