Elastix WeightedCombinationTransform

Beginner Questions
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1

Hi Everyone,

I’m very new to ITK and elastix. I have a set of 2-channel images of Tetraspeck fiducial beads in green and far red fluorescence and would like to register the two channels relative to each other for subsequent correction of chromatic aberration.

Can I average many transformations derived from registering pairs of images? I found these two classes WeightedCombinationTransform and WeightedCombinationTransformElastix, but couldn’t figure out how to use them. Could anyone kindly show me some example codes in Python?

Alternatively, is it possible to directly register multiple 2-channel images (i.e. registering a 2-channel stack)?

Thanks!

2

Maybe @Niels_Dekker could help? Meanwhile, you could take a look at registration metric for rgb image.

3

Thanks! @dzenanz Let me take a look