# Slice to Volume registration
**URL:** https://discourse.itk.org/t/slice-to-volume-registration/3659
**Category:** Uncategorized
**Created:** [November 26, 2020, 2:15pm UTC](https://discourse.itk.org/t/slice-to-volume-registration/3659 "2020-11-26T14:15:24Z")
**Posts on this page:** 4
**Page:** 1
### Author:  [@aabbcc](https://discourse.itk.org/u/aabbcc)
#### Post date: [November 26, 2020, 2:15pm UTC](https://discourse.itk.org/t/slice-to-volume-registration/3659/1 "2020-11-26T14:15:24Z")
I have a 3D microCT image about a tissue sample. And I use a ultramicrotome to get some ultrathin sections(slices) of the tissue. Then I get these slices’ 2D images using electron microscope. How can I use itk to register these 2D images to the 3D image in python. I am new in itk.
The 2D slice images are independent, so just one 2D image registers to the 3D image every time.
Any suggestions will be helpful.
Thanks
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### Author:  [@dzenanz](https://discourse.itk.org/u/dzenanz)
#### Post date: [November 26, 2020, 4:49pm UTC](https://discourse.itk.org/t/slice-to-volume-registration/3659/2 "2020-11-26T16:49:58Z")
Take a look at [relevant repositories](https://github.com/search?q=itk+2d+3d+registration&type=repositories) on GitHub. Also, [TwoProjectionRegistration](https://github.com/InsightSoftwareConsortium/ITKTwoProjectionRegistration) might be of interest. Look in the wrapping directory to see which classes are available via Python interface, e.g. [TwoProjection](https://github.com/InsightSoftwareConsortium/ITKTwoProjectionRegistration/tree/master/wrapping).
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### Author:  [@blowekamp](https://discourse.itk.org/u/blowekamp)
#### Post date: [November 27, 2020, 12:49pm UTC](https://discourse.itk.org/t/slice-to-volume-registration/3659/3 "2020-11-27T12:49:03Z")
@dzenanz Those repositories look like projected 2D to 3D, that is for use cases such as matching X-ray to CT. Are there any specific solutions that you are aware of that for a 3D single slice to a 3D volume?
@aabbcc Are the EM images acquired with scanning electron microscopy (SEM)?
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### Author:  [@aabbcc](https://discourse.itk.org/u/aabbcc)
#### Post date: [November 28, 2020, 2:06pm UTC](https://discourse.itk.org/t/slice-to-volume-registration/3659/4 "2020-11-28T14:06:35Z")
@blowekamp Yes!